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Scanning electron microscopy and X-ray microanalysis of foreign bodies associated with silastic implants in humans

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      Abstract

      Polydimethylsiloxane (Silastic) capsules containing megestrol acetate have been implanted in subcutaneous tissue as a method of long-term contraception. Histological studies revealed a granulomatous foreign body reaction around these capsules with birefringent crystals in multinucleated giant cells. Although several authors had interpreted these crytals as steroids, this seemed unlikely since the tissue had been processed with organic solvents. Analysis of these crystals by polarization microscopy, scanning electron microscopy, and X-ray microanalysis demonstrated that the material was talc. The talc was probably introduced as a contaminant from gloves during the implantation. Further analysis showed that capsule fragmentation could not have produced the material since Silastic particles could not be detected.
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      References

        • Kincl FA
        • Bengiano G
        • Angee I
        Sustained release hormonal preparations. I. Diffusion of various steroids through polymer membranes.
        Steroids. 1968; 11: 673-680
        • Ermini M
        • Carpino F
        • Fusso M
        • Benagiano G
        Studies on sustained contraceptive effects with subcutaneous polydimethysiloxane implants.
        Acta Endocrinologica. 1973; 73: 360-373
        • Pickett JP
        • Ingram P
        • Shelburne JD
        Identification of inorganic particulates in a single histologic section using both light microscopy and X-ray microprobe analysis.
        J of Histotechnology. 1980; 3: 155-158
      1. Shelburne JD, Ingram P, Hawkins HK: X-ray mapping of lysosomal inclusions. Scanning Electron Microscopy/1976 (Part III), I.I.T. Research Institute, Chicago, Ill., pp. 485–492

      2. Masters SK, Bell SW, Ingram P: Preparative techniques for freezing and freeze-sectioning macrophages for energy dispersive X-ray microanalysis. Scanning Electron Microscopy/1979/III, Johari O, Editor. SEM Inc., AMF O'Hare, Ill., 60666, pp. 97–110 and 122