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Research Article| Volume 13, ISSUE 2, P131-139, February 1976

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Processing of the preparations of β-subunit of human chorionic gonadotropin for minimization of cross-reactivity with human luteinizing hormone

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      Abstract

      β-HCG preparations demonstrate microheterogeneity and are resolved in multiple bands on electrophoresis in polyacrylamide gels. The gels were cut into segments and the immunological reactivity of eluates with anti-gb-HCG and anti-HLH sera was investigated. The bands reacting with the two antigonadotropin sera were identified. Graded absorption of β-HCG with anti-heterologous (ovine) LH gave preparations with none or minimal cross-reactivity with anti-HLH.
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