Research Article| Volume 13, ISSUE 2, P141-151, February 1976

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Immunological properties of dinitrophenyl derivatives of beta-subunit of the human chorionic gonadotropin

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      Four different derivatives with varying proportions of dinitrophenyl (DNP) groups tagged covalently to β-subunit of HCG (DNP(1)-β-HCG, DNP(3.3)-β-HCG, DNP(6)-β-HCG and DNP (10)-β-HCG) have been prepared. Their immunogenicity as well as the reactivity of antibodies formed in mice, rabbit and rhesus monkey have been studied. The derivatives give rise to the formation of antibodies in all species tested which react with HCG and in some species with DNP moieties. The anti-DNP response increases with derivatives bearing more than 3–4 DNP groups per molecule of β-HCG. On the other hand the anti-HCG response diminishes with derivatives bearing higher than 3–4 DNP groups per β-HCG molecule. The antibody response in monkeys is of long duration (> 14 months). The association constants for binding with HCG are of the order of 4 × 109 to 6 × 1010 L/mole. the affinity of the antibodies tends to increase with time.
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        • Weigle W.D.
        Immunological unresponsiveness.
        Advances in Immunology. 1973; 16: 100
        • Vaitukaitis J.L.
        • Robbins J.B.
        • Nieschlag E.
        • Ross G.T.
        Specific antisera with small doses of immunogen.
        J. Clin. Endocrinol. Metab. 1971; 33: 988-991
        • Rodbard D.
        Receptors for reproductive hormones. IIIrd edition. Adv. Exptl. Med. & Biol. Plenum Publishing Corp, N.Y1973: 289-326
        • Scatchard G.
        The attractions of proteins for small molecules and ions.
        Ann. N.Y. Acad. Sci. 1949; 51: 660-672
        • Carlson R.B.
        • Bahl O.P.
        • Swaminathan N.
        Human chorionic gonadotropin. Linear aminoacid sequence of the β-subunit.
        J. Biol. Chem. 1973; 248: 6810-6827
        • Morgan F.J.
        • Birken S.
        • Canfield R.E.
        Chemistry of human chorionic gonadotropin.
        in: Moudgal N.R. Gonadotropins and gonadal function. Acad. Press, N.Y1974: 79-92