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Abstract
Four different derivatives with varying proportions of dinitrophenyl (DNP) groups
tagged covalently to β-subunit of HCG (DNP(1)-β-HCG, DNP(3.3)-β-HCG, DNP(6)-β-HCG and DNP (10)-β-HCG) have been prepared. Their immunogenicity as well as the reactivity of antibodies
formed in mice, rabbit and rhesus monkey have been studied. The derivatives give rise
to the formation of antibodies in all species tested which react with HCG and in some
species with DNP moieties. The anti-DNP response increases with derivatives bearing
more than 3–4 DNP groups per molecule of β-HCG. On the other hand the anti-HCG response
diminishes with derivatives bearing higher than 3–4 DNP groups per β-HCG molecule.
The antibody response in monkeys is of long duration (> 14 months). The association
constants for binding with HCG are of the order of 4 × 109 to 6 × 1010 L/mole. the affinity of the antibodies tends to increase with time.
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Article info
Publication history
Accepted:
October 20,
1975
Identification
Copyright
© 1976 Published by Elsevier Inc.
