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Abstract
Processed beta-subunit of HCG conjugated covalently to tetanus toxoid (Pr-β-HCG-TT)
was found to be immunogenic in a number of species of animals tested. Single or multiple
injections of the conjugate were required depending on the adjuvant used. The conjugate
gives rise to the concomitant formation of antibodies against both HCG and tetanus
toxoid. In rhesus monkeys, the antibodies appeared after a lag period of a few weeks,
and increased progressively to attain plateau levels anywhere between 9 and 20 weeks.
The duration of antibody response after primary immunization in most monkeys was beyond
one year. The response was reversible and antibody titers declined to insignificantly
low levels in course of time. A single repeat injection was adequate to evoke a powerful
secondary immune response. The conjugate elicits the formation of antibodies with
fairly high affinity constants for binding with HCG. Ka values ranged from 109 to 1010 L/mole.
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References
Talwar, G.P. Sharma, N.C., Dubey, S.K., Salahuddin, M., Das, C., Ramakrishnan, S., Kumar, S. and Hingorani, V.: Isoiramunization against human chorionic gonadotropin with conjugates of processed β-subunit of the hormone and tetanus toxoid. Proc. Natl. Acad. Sci. (in Press)
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- Production of antisera for radioimmunoassays.in: Odell W.D. Daughaday W.H. Principles of competitive protein-binding assays. J.B. Lippincott Co, Philadelphia1971: 57-88
- Immunological properties of dinitrophenyl derivatives of beta-subunit of the human chorionic gonadotropin.Contraception. 1976; 13: 141
Article info
Publication history
Accepted:
October 20,
1975
Identification
Copyright
© 1976 Published by Elsevier Inc.
