Research Article| Volume 13, ISSUE 2, P163-170, February 1976

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Immunological reactivity of antibodies produced by Pr-β-HCG-TT with different hormones

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      Antisera produced by injection of Pr-β-HCG-TT in rhesus monkeys (Math Eq) and goat have been analysed for reactivity with various hormones. All antisera bound 125I-HCG. β-HCG competed with labelled HCG for binding to the sera in all cases. No cross-reactivity of human placental lactogen (HPL) and human growth hormone (HGH) was seen up to 1μg quantities per assay tube as gauged by competitive inhibition assays. Human follicle stimulating hormone (HFSH), human luteinizing hormone (HLH) and human thyroid stimulating hormone (HTSH) did not compete with 125I-HCG at the normal maximum physiological concentration of these hormones. The concentrations in radioimmunoassay system at which the competition appears with other hormones have also been determined.
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