Advertisement

Flowers of Hibiscus rosa-sinensis, a potential source of contragestative agent. III: Interceptive effect of benzene extract in mouse

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      In mouse, oral administration of the benzene extract of Hibiscus rosa-sinensis flowers at a dose level of 1 gm/kg body weight/day 5–8 of gestation led to termination of pregnancy in about 92% of the animals. The effect was associated with a significant fall in peripheral level of progesterone and increase in uterine acid phosphatase activity, as measured on day 10. The ovary exhibited signs of luteolysis, and the corpus luteal Δ5-3β-hydroxysteroid dehydrogenase activity decreased markedly. The interceptive effect of the extract was prevented completely by exogenous progesterone (1 mg/mouse/day) or chorionic gonadotropin (1 1.μ./mouse/day) and partially (62.5%) by exogenous prolactin (500 ug/mouse/day). In unilaterally pregnant mouse having traumainduced deciduomata in the sterile horn, the extract caused resorption of the fetuses, and regression of the deciduomata accompanied by reduction in weight of the ovaries. Luteolysis, may be due to interference with the luteotropic influence, and a consequent fall in plasma level of progesterone have been suggested as the plausible cause of termination of pregnancy.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Contraception
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Mahli B.S.
        • Trivedi V.P.
        Vegetable antifertility drugs of India.
        Quart. J. Crude Drug Res. 1972; 12: 1922-1923
        • Holdsworth D.K.
        Medicinal plants of Papua-New Guinea, Noumea, New Caledonia.
        South Pacific Commission Technical Paper no. 175. 1977;
        • Dixit V.P.
        Effect of chronically administered Malvaviscus flower extract on the female genital tract.
        Indian J. Exp. Biol. 1977; 15: 650-651
        • Batta S.K.
        • Santhakumari G.
        The antifertility activity of Ocimum sanctum and Hibiscus rosa-sinensis.
        Indian J. Med. Res. 1971; 59: 777-781
        • Kabir S.N.
        • Bhattacharya K.
        • Pal A.K.
        • Pakrashi A.
        Flowers of Hibiscus rosa-sinensis, a potential source of contragestative agent: I. Effect of benzene extract on implantation of mouse.
        Contraception. 1984; 29: 385-397
        • Pal A.K.
        • Bhattacharya K.
        • Kabir S.N.
        • Pakrashi A.
        Flowers of Hibiscus rosa-sinensis, a potential source of contragestative agent: II. Possible mode of action with reference to anti-implantation effect of benzene extract.
        Contraception. 1985; 32: 517-529
        • Lawrence Jr., I.E.
        • Burden H.W.
        • Louis T.M.
        Effect of abdominal vagotomy of the pregnant rat on LH and progesterone concentrations and fetal resorption.
        J. Reprod. Fert. 1978; 53: 131-136
        • Bessey O.A.
        • Lowry O.H.
        • Brock M.J.
        J. Biol. Chem. 1946; 164: 321
      1. (Cited in)
        • Bessey O.A.
        • Lowry O.H.
        • Brock M.J.
        Methods of Enzymatic Analysis.
        in: Bergmeyer H.U. Academic Press, New York1965: 783-785
        • Segal I.H.
        Biochemical Calculations.
        in: 2nd ed. John Wiley and Sons, New York1976: 334
        • Brotherton J.
        Sex Hormone Pharmacology. Contraception. Academic Press, New York1976: 198-247
        • De Feo V.J.
        Decidualization.
        in: Wynn R.M. Cellular Biology of the Uterus. North Holland Publishing Co, Amsterdam1967: 191-290
        • Orczyk G.P.
        • Hichens M.
        • Arth G.
        • Behrman H.R.
        Progesterone.
        in: Jaffe B.M. Behrman H.R. Methods of Hormone Radioimmunoassay. Academic Press, New York1974: 347-358
        • Pal A.K.
        • Kabir S.N.
        • Pakrashi A.
        A probe into the possible mechanism underlying the interceptive effect of aristolic acid.
        Contraception. 1982; 25: 639-648
        • Aitken R.J.
        The hormonal control of implantation.
        in: Maternal Recognition of Pregnancy. Ciba Foundation Symposium. 64. 1979: 53-83
        • Dickman Z.
        • Dey S.K.
        • Sengupta J.
        A new concept: Control of early pregnancy by steroid hormones originating in the pre-implantation embryo.
        in: Vitamins and Hormones. Vol. 34. Academic Press, Orlando, Florida1976: 215-242
        • Smith J.G.
        • Hanks J.
        • Short R.V.
        Biochemical observations on the corpora lutea of the African elephant Loxodonta African.
        J. Reprod. Fert. 1969; 20: 111-117
        • Ryan K.J.
        Steroid hormones in mammalian pregnancy.
        in: Handbook of Physiology. Vol. II. American Physiological Society, Washington D.C1973: 285-293
        • Leavitt W.W.
        • Chen J.J.
        • Allen T.C.
        Regulation of progesterone receptor formation by estrogen action.
        Ann.N.Y.Acad.Sci. 1977; 286: 210-225
        • Rao R.B.
        • Wiest W.G.
        • Allen W.M.
        Progesterone receptor in rabbit uterus. I. Characterization and estradiol-17β augmentation.
        Endocrinol. 1973; 92: 1229-1240
        • Murr S.M.
        • Stabenfeldt G.H.
        • Bradford G.E.
        • Geschwind I.I.
        Plasma progesterone during pregnancy in the mouse.
        Endocrinology. 1974; 94: 1209-1213
        • Choudary J.B.
        • Greenwald G.S.
        Ovarian activity in the intact or hypophysectomized pregnant mouse.
        Anat. Rec. 1969; 163: 359-372
        • Weist W.G.
        • Kidwell W.R.
        The regulation of progesterone secretion by ovarian dehydrogenases.
        in: The Gonads. Appleton, New York1969: 295-325
        • Choudary J.B.
        • Greenwald G.S.
        Luteotropic complex of the mouse.
        Anat. Rec. 1969; 163: 373-388
        • Critser E.S.
        • Rutledge J.J.
        • French L.R.
        Role of the uterus and the conceptus in regulating luteal life span in the mouse.
        Biol.Reprod. 1980; 23: 558-563
        • Rarkley M.S.
        The temporal relationship between implantation, termination of prolactin surges and increased secretion in the pregnancy.
        Endocrinology. 1980; 23: 558-563
        • Purandare T.V.
        • Dattatreymurty B.
        • Munshi S.R.
        • Rao S.S.
        Immunobiological actions of antiserum to ovine prolactin in mouse.
        J. Reprod. Fert. 1975; 44: 411-419
        • Mednick D.L.
        • Berkley M.S.
        • Geschwind I.I.
        Regulation of progesterone secretion by LH and prolactin during first half of pregnancy in the mouse.
        J. Reprod. Fert. 1980; 60: 201-207
        • Armstrong D.T.
        • Knudsen K.A.
        • Miller L.S.
        Effect of prolactin upon cholesterol metabolism and progesterone biosynthesis in corpora lutea of rats hypophysectomized during pseudopregnancy.
        Endocrinology. 1970; 86: 634-641
        • Morishige W.K.
        • Rothchild I.
        Temporal aspects of the regulation of corpus luteum function by luteinizing hormone, prolactin and placental luteotrophin during the first half of pregnancy in the rat.
        Endocrinology. 1974; 95: 1209-1213