Research Article| Volume 34, ISSUE 5, P537-544, November 1986

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Effects of momorcharins on the mouse embryo at the early organogenesis stage

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      Previous studies showed that the two forms of momorcharins which are isolated from seeds of Math Eq L. are effective in inducing early and midterm abortions in the mouse. Momorcharins were found to be teratogenic to the cultured mouse embryos at the early organogenesis stage. Morphological abnormalities were seen in the head, trunk and limbs. Ultrastructural studies on the visceral yolk sac of the momorcharin-treated embryos showed that the endodermal layer was deranged, membrane invaginations at the apical surface were decreased and intercellular space became distended. It is likely that the teratogenic action of momorcharins on mouse embryos Math Eq is mediated through the deleterious effects on the visceral yolk sac, which functions as a vital transport organ for the conceptus at the immediate post-implantation period.
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        • Yeung H.W.
        • Li W.W.
        • Law L.K.
        • Chan W.Y.
        Purification and partial characterization of momorcharins, abortifacient proteins from the Chinese drugs, Kuguazi.
        in: Chang H.M. Yeung H.W. Tso W.W. Koo A. Advances in Chinese Medicinal Materials Research. World Scientific Publishing Co, 1985: 311-318
        • Chan W.Y.
        • Tam P.P.L.
        • Yeung H.W.
        The termination of early pregnancy in the mouse by β-momorcharin.
        Contraception. 1984; 29: 91-100
        • Chan W.Y.
        Biochemical characterization and biological effects of β-momorcharin, a new abortifacient protein from the Chinese herb Kuguazi.
        in: M.Phil.thesis. The Chinese University of Hong Kong, 1984
        • Law L.K.
        • Tam P.P.L.
        • Yeung H.W.
        Effects of α-trichosanthin and α-momorcharin on the development of peri-implantation mouse embryos.
        J. Reprod. Fert. 1983; 69: 597-604
        • Law L.K.
        α-momorcharin, a new abortifacient protein from the Chinese drug Kuguazi — purification and effect on early pregnancy in the mouse.
        in: M.Phil. thesis. The Chinese University of Hong Kong, 1983
        • Tam P.P.L.
        • Chan W.Y.
        • Yeung H.W.
        Effects of two forms of momorcharins and alpha-trichosanthin on early pregnancy in the mouse.
        in: Chang H.M. Yeung H.W. Tso W.W. Koo A. Advances in Chinese Medicinal Materials Research. World Scientific Publishing Co, 1985: 335-350
        • Chan W.Y.
        • Tam P.P.L.
        • Yeung H.W.
        The inhibitory effects of β-momorcharin on endometrial cells in the mouse.
        Contraception. 1985; 31: 83-90
        • Ooi V.E.C.
        • Shum L.K.W.
        • Yeung H.W.
        SEM studies on termination of abortion of the rat by an extract from Math Eq seeds.
        in: Proc. XIth Int. Cong. on Electron Microscopy, Kyoto1986: 3597-3598
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 165-175
        • Marks P.A.
        • Rifkind R.A.
        • Bank A.
        Erythroid cell differentiation.
        in: Paul J. Biochemistry of Cell Differentiation. Univ. Park Press, Baltimore1974: 129-160
        • Dziadek M.
        • Adamson E.
        Localization and synthesis of alpha-fetoprotein in post-implantation mouse embryos.
        J. Embryol. Exp. Morph. 1978; 43: 289-313
        • Gitlin D.
        • Boesman M.
        Fetus-specific serum proteins in several mammals and their reaction to human alpha-fetoprotein.
        Comp. Biochem. Physiol. 1967; 21: 327-336
        • Gustine D.L.
        • Zimmerman E.F.
        Amniotic fluid proteins: evidence for the presence of plasma glycoproteins in mouse amniotic fluid.
        Amer. J. Obstet. Gynecol. 1972; 114: 553-560
        • Freeman S.J.
        • Brent R.L.
        • Lloyd J.B.
        The effect of teratogenic antiserum on yolk sac function in rat embryos cultured Math Eq.
        J. Embryol. exp. Morph. 1982; 71: 63-74
        • Freeman S.J.
        • Lloyd J.B.
        Evidence that protein ingested by the rat visceral yolk sac yields amino acids for synthesis of embryonic protein.
        J. Embryol. exp. Morph. 1983; 73: 307-315
        • Jollie W.P.
        • Triche T.J.
        Ruthenium labelling of micropinocytotic activity in rat visceral yolk-sac placenta.
        J. Utrastruct. Res. 1971; 35: 541-553
        • Freeman S.J.
        • Brown N.A.
        An Math Eq study of teratogenicity in the rat due to antibody-induced yolk sac dysfunction: identification of the yolk sac antigen involved.
        Roux's Arch Dev. Biol. 1986; 195: 236-242
        • Kenis M.M.
        • Johnson E.M.
        Effects of trypan blue and Niagara Blue 2B on the Math Eq absorption of ions by the rat visceral yolk sac.
        J. Embryol. exp. Morph. 1969; 22: 115-125
        • Leung C.C.K.
        • Lee C.
        • Cheewatrakoolpong B.
        • Hilton D.
        Abnormal embryonic development induced by antibodies to rat visceral yolk sac endoderm: isolation of the antigen and localization to microvillar membrane.
        Devl. Biol. 1985; 107: 432-441
        • Barbieri L.
        • Zamboni M.
        • Lorenzoni E.
        • Montanaro L.
        • Sperti S.
        • Stirpe F.
        Inhibition of protein synthesis Math Eq by proteins from seeds of Math Eq (bitter pear melon).
        Biochem. J. 1980; 186: 443-452
        • Gasperi-Campani A.
        • Barbieri L.
        • Morelli P.
        • Stirpe F.
        Seed extracts inhibiting protein synthesis Math Eq.
        Biochem. J. 1980; 186: 439-441
        • Olsnes S.
        • Phil A.
        The specificity and action of animal, bacterial and plant toxins.
        in: Receptors and Recognition, Series B. Vol 1. Chapman and Hall, London1976: 129-173
        • Stirpe F.
        • Olsnes S.
        • Phil A.
        Gelonin, a new inhibitor of protein synthesis, nontoxic to intact cells.
        J. Biol. Chem. 1980; 225: 6947-6953
        • Janzen R.G.
        • Andrews G.K.
        • Tamaoki T.
        Synthesis of secretory proteins in developing mouse yolk sac.
        Devl. Biol. 1982; 90: 18-23
        • Young M.F.
        • Minghetti P.P.
        • Klein N.W.
        Yolk sac endoderm: exclusive site of serum protein synthesis in the early chick embryo.
        Devl. Biol. 1980; 75: 239-245