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Research Article| Volume 36, ISSUE 5, P549-555, November 1987

The effect of RU486 on transport, development and implantation of mouse embryos

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      Abstract

      Pregnancy was interrupted in Swiss-Rockefeller mice by a single subcutaneous injection of the antiprogesterone RU486 given postcoitally. A dose of 0.1 mg/animal injected on day 1, produced partial inhibition of pregnancy, a notable delay in embryonic development and a slight retention of embryos in the oviducts. When the same dose was injected on day 2, 3 or 4, no implantation sites were seen at autopsy performed on day 12 of pregnancy. Treatment with 0.5 or 1 mg/animal on day 1 produced the loss of a large proportion of the embryos from the genital tract by day 4 of pregnancy and suppressed implantation completely. These observations indicate that preimplantation phenomena in mice are highly dependent on progesterone action.
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      References

        • Kendle K.E.
        • Lee B.
        Investigation of the influence of progesterone on mouse embryo transport by using antiprogestational steroid.
        J Reprod Fert. 1980; 58: 253
        • Wang M.Y.
        • Rider V.
        • Heap R.B.
        • Feinstein A.
        Action of anti-progesterone monoclonal antibody in blocking pregnancy after postcoital administration in mice.
        J Endocr. 1984; 101: 95
        • Philibert D.
        • Moguilewsky M.
        • Mary I.
        • Lecaque D.
        • Tournemine C.
        • Secchi J.
        • Deraedt T.
        Pharmacological profile of RU486 in animals.
        in: Balieu E-E. Segal S.J. The Antiprogestin Steroid RU486 and Human Fertility Control. Plenum Press, New York1985: 49-68
        • Psychoyos A.
        Uterine receptivity for nidation.
        Ann NY Acad Sc. 1986; 476: 36
        • Tarkowski A.K.
        An air-drying method for chromosome preparations from mouse eggs.
        Cytogenetics. 1966; 5: 394
        • Roblero L.
        • Garavagno A.
        Effect of oestradiol-17B and progesterone on oviductal transport and early development of mouse embryos.
        J Reprod Fert. 1979; 57: 91
        • Roblero L.
        • Moscoso H.
        • Riffo M.
        Effect of postcoital oestradiol treatment upon transport, growth, differentiation and viability of preimplantation mouse embryos.
        Arch Biol Med Exp. 1983; 16: 55