Research Article| Volume 36, ISSUE 5, P567-580, November 1987

A new non-hormonal antifertility drug DL-204: II. Effect on testicular hyaluronidase and gamma-glutamyl transpeptidase in male rats

      This paper is only available as a PDF. To read, Please Download here.


      Effects of DL-204, 2-(3-ethoxyphenyl)-5,6-dihydro (5,1-a)-isoquinoline, a non-hormonal antifertility drug on testicular hyaluronidase and gammaglutamyl transpeptidase levels, biochemical markers for testicular function, were evaluated in male rats. Treatment of 21-day-old rats with DL-204 for 7 or 15 days produced cryptorchid condition. Testicular hyaluronidase and gamma-glutamyl transpeptidase levels reveal that DL-204 acts on the testes, possibly in two ways; one, by reducing the gonadotropin levels thereby reducing the levels of androgens as reflected by reduced accessory reproductive organ weights and, secondly, by a direct action on the testes. Thus, we conclude that DL-204 is acting as an antispermatogenic agent, possibly acting in more than one way on the testes.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Contraception
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Galliani G.
        • Gallico L.
        • Cattaneo C.
        • Lerner L.J.
        Pregnancy termina- tion in various species by 2(3-ethoxyphenyl)-5,6-dihydro-S-triazole (5,I-a) isoquinoline (DI-204-IT or L-11204).
        Acta Endocrinol. 1977; 85: 126
        • Lerner L.J.
        • Omodei-Sale A.
        • Galliani G.
        • Geywood R.
        • Hall J.M.
        • Grant A.M.
        A new class of pregnancy terminating agents effective in primates.
        Fertil. Steril. 1977; 28: 290
        • Galliani G.
        • Lerner L.J.
        • Caramel C.
        • Maraschin R.
        • Nani S.
        • Nava A.
        Pregnancy termination in dogs with novel non-hormonal compounds Studies of 2-(3-ethoxypheny)5,6-dihydro-S-triazole (5,I-a) isoquinoline (DL-204-IT).
        Arzeneim. Forsch. Drug Res. 1982; 32: 123
        • Prasad R.S.
        • Vijayan E.
        A new non-hormonal anti-fertility drug DL-204: I. Effects on testes and accessory glands of reproduction in male rats.
        Contraception. 1987; 36: 557
        • Prasad R.S.
        • Vijayan E.
        A new non-hormonal antifertility drug DL-III-IT: I Effects on testes and accessory glands of reproduction in male rats.
        Contraception. 1986; 33: 79
        • Prasad R.S.
        • Vijayan E.
        A non-hormonal antifertility drug DL-III-IT: II, Effects on testicular hyaluronidase activity in male rats.
        Contraception. 1986; 33: 89
        • Males J.L.
        • Turkington R.W.
        Hormonal regulation of hyaluronidase during spermatogenesis in the rat.
        J. Biol. Chem. 1970; 245: 6329
        • Doak G.A.
        • Zahler W.L.
        Stimulation of bull sperm hyaluronidase by poly cations.
        Biochem. Biophys. Acta. 1979; 570: 303
        • Tate S.S.
        • Meister A.
        Interaction of gamma-glutamyl transpeptidase with amino acids, dipeptides and derivatives and analogs of glutathione.
        J. Biol. Chem. 1974; 249: 7593
        • Sadasivudu B.
        • Rao G.N.
        • Swamy M.
        Gamma-glutamyl transpeptidase and gamma-glutamyl cysteine synthetase activity in human lens tissue.
        IRCS Med. Sci. 1981; 9: 55
        • Lowry O.H.
        • Rosebrough M.J.
        • Farr A.L.
        • Randall R.
        Protein measurement with Folin-phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • Steinberger E.
        Hormonal control of mammalian spermatogenesis.
        Physiol. Rev. 1971; 51: 1
        • Chowdhury A.K.
        • Steinberger E.
        The influence of cryptorchid milieu on the initiation of spermatogenesis in the rat.
        J. Reprod. Fertil. 1972; 29: 173
        • Chowdhury A.K.
        • Steinberger E.
        Effect of 5 α-reduced androgens on sex accessory organs and initiation and maintenance of spermatogenesis in the rat.
        Biol. Reprod. 1975; 12: 609
        • Hansson V.
        • French F.S.
        • Weddington S.C.
        • Nayfeh S.N.
        • Ritzen E.M.
        FSH stimulation of testicular androgen binding protein (ABP)-androgen priming increases the response to FSH.
        in: Lipsett M. Hormone Binding and Target Cell Activation in the testes. Plenum Press, N.Y1974: 287
        • Means A.R.
        • Fakunding J.L.
        • Huckins D.J.
        • Tindall D.J.
        • Vitale R.
        Follicle stimulating hormone, the Sertoli cell and spermatogenesis.
        Rec. Prog. Horm. Res. 1976; 32: 477
        • Sharpe R.M.
        Local control of testicular function.
        Q. J. Exp. Physiol. 1983; 68: 265
        • Madhwaraj H.G.M.
        • Dym M.
        The effect of selective withdrawal of FSH or LH on spermatogenesis in the immature rat.
        Biol. Reprod. 1976; 14: 489
        • Delaap L.W.
        • Tate S.S.
        • Meister A.
        Gamma-glutamyl transpeptidase and related enzymes in the reproductive system of the male rat.
        Life Sciences. 1977; 20: 673
        • Hodgen G.D.
        • Sherins R.J.
        Enzymes as markers of testicular growth and development in the rat.
        Endocrinology. 1973; 93: 985
        • Krueger P.M.
        • Hodgen G.D.
        • Sherins R.J.
        New evidence for the role of the Sertoli cell and spermatogonia in feedback control of FSH secretion in male rats.
        Endocrinology. 1974; 95: 955
        • Lu C.
        • Steinberger E.
        Gamma-glutamyl transpeptidase activity in developing rat testis. Enzyme localization in isolated cell types.
        Biol. Reprod. 1977; 17: 84
        • Chemes H.E.
        • Dym M.
        • Raj H.G.M.
        The role of gonadotropins in initiation of spermatogenesis in the immature rat.
        Biol. Reprod. 1979; 21: 241
        • Dym M.
        • Raj H.G.M.
        Response of adult rat Sertoli cells and Leydig cells to depletion of LH and testosterone.
        Biol. Reprod. 1977; 17: 676
        • Kula K.
        The influence of human menopausal gonadotropin natrium fluoride and cyproterone acetate on the spermatogenesis in immature rats.
        Andrologia. 1978; 10: 223
        • Libbus B.L.
        • Scheutz A.W.
        Gonadotropin-induced reinitiation of meiosis in testis of oestrodiol-treated prepubertal rats.
        J. Reprod. Fertl. 1980; 60: 11
        • Sharpe R.M.
        Intratesticular factors controlling testicular function.
        Biol. Reprod. 1984; 30: 29
        • Sherins R.J.
        • Hodgen G.D.
        Testicular gamma glutamyl-transpeptidase an index of Sertoli cell function in man.
        J. Reprod. Fertil. 1976; 48: 191
        • Galdieri M.
        • Zani B.M.
        • Monaco L.
        • Ziparo E.
        • Steffanini M.
        Changes of Sertoli cell glycoproteins induced by removal of the associated germ cells.
        Exp. Cell. Res. 1983; 145: 191
        • Galdieri M.
        • Monaco L.
        Sertoli cell membrane polypeptide composition is modulated by germ cells.
        Cell Differentiation. 1986; 18: 51