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Spermicidal activity of an antifungal saponin obtained from the tropical herb Mollugo pentaphylla

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      Abstract

      The ethyl acetate fraction of Mollugo pentaphylla, a tropical herb, contains an antifungal saponin (mollugogenol-A). We report here the spermicidal effects of this saponin. Washed sperm (> 100 × 106with > 50% motility) from normal volunteers were incubated with varying concentrations (0–300μg/ml) of mollugogenol-A at 30 °C. Sperm motility, velocity and viability were assessed at 0, 30, 60 minutes both manually and by using computer assisted semen analysis (CASA). Samples collected at 0 and 60 minutes were evaluated for membrane lipid peroxidation, superoxide dismutase (SOD) activity and transmission electron microscopy.
      A dose- and time-dependent effect of this saponin on sperm motion and viability was observed. The maximal spermicidal effect (4–5 fold decrease in motility and viability) was observed with 300 μg/ml dose of saponin. A three-fold increase in sperm membrane lipid peroxidation with corresponding inhibition of SOD activity were observed after 60 minutes incubation with this spermicidal agent. Transmission electron microscopy of saponin-treated samples revealed significant damage to the sperm membrane in both head and tail regions, and the acrosomal membranes were notably swollen and disrupted. These results indicate that this natural saponin has a potential spermicidal effect besides its known antifungal activity. The likely mechanism of its action involves sperm membrane damage by increased lipid peroxidation.
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      References

        • Hamburger M
        • Dudan G
        • Nair AGR
        • Jayapraksam R
        • Hostettmann K
        An antifungal triterpenoid from Mollugo pentaphylla.
        Phytochemistry. 1989; 28: 1767-1768
        • Mahato SB
        • Sarkar SK
        • Poddar G
        Triterpenoid saponins.
        Phytochemistry. 1988; 27: 3037-3067
        • Heftman E
        Biochemistry of steroid saponins and glycoalkaloids.
        Lloydia. 1967; 30: 209-230
        • Ishihama A
        • Inoue T
        Clinical field tests of a new contraceptive vaginal foam tablet.
        Contraception. 1972; 6: 401-404
        • Feldblum PJ
        • Bernardik E
        • Rosenberg MJ
        Spermicide use and sexually transmitted disease.
        JAMA. 1988; 259: 2851
        • Sikka SC
        • Hellstrom WJG
        The application of pentoxifylline in the stimulation of sperm motion undergoing electroejaculation.
        J Androl. 1991; 12: 165-170
        • Eliasson R
        Supravital staining of human spermatozoa.
        Fertil Steril. 1977; 28: 1257
        • Rao B
        • Soufir JC
        • Martin M
        • David G
        Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility.
        Gamete Res. 1989; 24: 127-134
        • Kobayashi T
        • Miyazaki T
        • Natori M
        • Nozawa S
        Protective role of superoxide dismutase in human sperm motility: Superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa.
        Human reproduction. 1991; 6: 987-991
        • Oyanagui Y
        Reevaluation of assay methods and establishment of kit for superoxide dismutase activity.
        Anal Biochem. 1984; 142: 290-296
        • Pakrashi A
        • Ray H
        • Pal BC
        • Mohato SB
        Sperm immobilizing effect of triterpene saponins from Acacia auriculiformis.
        Contraception. 1991; 43: 475-483
        • Setty BS
        • Kamboj VP
        • Garg HS
        • Khanna NM
        Spermicidal potential of saponins isolated from Indian medicinal plants.
        Contraception. 1976; 14: 571-578
        • Stolzenberg SJ
        • Parkrust RM
        Spermicidal actions of extracts and compounds from Phytolacca dodecandra.
        Contraception. 1974; 10: 135-143
        • Brotherton J
        Assesment of spermicides by a stripping technique against human spermatozoa.
        J Reprod Fert. 1977; 51: 383-391
        • Zamboni L
        Sperm structure and its relevance to infertility: An electron microscopic study.
        Arch Pathol Lab Med. 1992; 116: 325-344
        • Jones R
        • Mann T
        • Sherins RJ
        Oxidative breakdown of lipids in human spermatozoa, Spermicidal properties of fatty acid peroxides and protective action of seminal plasma.
        Fertil Steril. 1979; 31: 531-537
        • Alvarez JG
        • Storey BT
        Spontaneous lipid peroxidation in rabbit epididymal spermatozoa.
        Biol Reprod. 1982; 27: 1102-1108
        • Alvarez JG
        • Storey BT
        Spontaneous lipid peroxidation in rabbit and mouse epididymal spermatozoa: dependence of rate on temperature and oxygen concentration.
        Biol Reprod. 1985; 32: 342-351
        • Riley JCM
        • Behrman RH
        Oxygen radicals and reactive oxygen species in reproduction.
        in: Proc Soc. Exp Biol Med. 198. 1991: 781-791
        • Kanwar U
        • Kaur R
        • Chadha S
        • Sanyal S
        Gossypol-induced inhibition of glucose uptake in human ejaculated spermatozoa may be mediated by lipid peroxidation.
        Contraception. 1990; 42: 573-587
        • Kovalski NN
        • de Lamirande E
        • Gagnon C
        Reactive oxygen species generated by human neutrophils inhibit sperm motility: Protective effect of seminal plasma and scavengers.
        Fertil Steril. 1992; 58: 809-816
        • Alvarez JG
        • Touchstone JC
        • Blasco L
        • Storey BT
        Spontaneous lipid peroxidation and production of hydrogen peroxide and Superoxide in human spermatozoa: Superoxide dismutase as major enzyme protectant against oxygen toxicity.
        J Androl. 1987; 8: 338-348
        • Alvarez JG
        • Storey BT
        Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation.
        J Androl. 1992; 13: 232-241