Research Article| Volume 49, ISSUE 2, P161-169, February 1994

Long-term contraceptive effects of intrauterine neem treatment (IUNT) in bonnet monkeys: An alternate to intrauterine contraceptive devices (IUCD)

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      Antifeitility effects of intrauterine neem treatment (IUNT) was studied in bonnet monkeys. A single administration of 1 ml of neem oil by an intrauterine insemination catheter blocked fertility for 7 to 12 months. The effect was, however, reversible as all the animals became pregnant subsequently and delivered normal babies. The neem oil treatment had no adverse effect on menstrual cyclicity and ovarian functions. The uterus of neem-treated animals showed normal morphology. Immunohistological studies, however, demonstrated a significant increase in the number of MHC-II antigen-positive cells in the uterine endometrium following neem treatment, indicating enhanced antigen-presenting ability of the uterus; a feature that may be related to the observed antifertility effect of neem oil. The present investigation demonstrates that an IUNT can be used for long-term, reversible contraception, without any apparent side effects, and that the method could provide an alternate to currently used intrauterine contraceptive devices (IUCD).


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      1. Randhawa NS Parmar BS Neem: Research and Development, Society of Pesticide Science, India. Indian Agricultural Research Institute,, New Delhi1993: 283
        • Rao AR
        • Kumar S
        • Paramsivam TB
        • Kamalakshi S
        • Parashuraman AR
        • Shantha M
        Study of anti-viral activity of tender leaves of Margosa tree (Melia azadirachta) on vaccinia and variola viruses: A preliminary report.
        Indian J Med Res. 1969; 57: 495-502
        • Gogati SS
        • Marathe AD
        Anti-viral effect of neem leaf (Azardirachta indica) extracts on chinkungunya and measles viruses.
        J Res Edu Indian Med. 1989; 8: 1-5
        • Chopra IC
        • Gupta KC
        • Nazir BN
        Preliminary study of anti-bacterial substances from Melia azaidirchta.
        Indian J Med Res. 1952; 40: 511-515
        • Singh N
        • Sastry MS
        Anti-microbial activity of neem oil.
        Indian J Pharmacol. 1981; 13: 102
        • Kher A
        • Chaurasia SC
        Anti-fungal activity of essential oils of three medicinal plants.
        Indian Drugs. 1997; 15: 41-42
        • Singh UP
        • Singh HB
        • Singh RB
        Effect of neem (Azadirachta indica) extracts on some soil-borne pathogens of gram (Cicer arietinum).
        Micologia. 1980; 72: 1077-1093
        • Sinha KC
        • Riar SS
        • Tiwary RS
        • et al.
        Neem oil as vaginal contraceptive.
        Indian J Med Res. 1984; 79: 131-135
        • Lal R
        • Sankaranarayana A
        • Mathur VS
        • Sharma PL
        Antifertility effect of neem oil in female albino rats by intravaginal and oral routes.
        Indian J Med Res. 1986; 83: 89-92
        • Riar SS
        • Bardhan J
        • Thomas P
        • Kain AK
        • Parshad R
        Mechanism of antifertility action of neem oil.
        Indian J Med Res. 1988; 88: 339-342
        • Upadhyay SN
        • Kaushic C
        • Talwar GP
        Antifertility effects of neem (Azadirchta indica) oil by single intrauterine administration: a novel method for contraception.
        in: Proc Roy Soc, London. 242. 1990: 175-179
        • Upadhyay SN
        • Dhawan S
        • Talwar GP
        Antifertility effects of neem (Azadi-rachta indica) oil in male rats by single intravas administration: an alternate approach to vasectomy.
        J Androl. 1993; 14: 275-281
        • Upadhyay SN
        • Dhawan S
        • Garg S
        • Talwar GP
        Immunomodulatory effects of neem (Azadirachta indica) oil.
        Intern J Immunopharmacol. 1992; 14: 1187-1193
        • Sufi SB
        • Donaldson A
        • Jeffcoate Sl
        Progesterone assay.
        in: WHO Method Manual. W.H.O.,, Geneva1989: 67-79
        • Zipper J
        • Medel M
        • Prager R
        Alterations in fertility induced by unilateral intrauterine instillation of cytotoxic compounds in rats.
        Am J Obstet Gynecol. 1968; 101: 971-978
        • Schwartz RH
        • Yano A
        • Paul WE
        Interaction between antigen-presenting cells and primed T-lymphocytes: an assessment of Ir gene expression in the antigen-presenting cell.
        Immunol Rev. 1978; 40: 153
        • Unanue ER
        The regulation of lymphocyte functions by the macrophage.
        Immunol Rev. 1978; 40: 227
        • Hunt JS
        • Manning LS
        • Wood GW
        Macrophages in murine uterus are immuno-suppressive.
        Cell Immunol. 1984; 85: 499-510
        • Witkin S
        • McGregor JA
        Infection-induced activation of CMI: possible mechanism for pre-term birth.
        Clinic Obstet Gynecol. 1991; 34: 112-122
        • Labadie RP
        • Van der Nat JM
        • Simons JM
        • et al.
        An ethanopharmacognostic approach to the search for immunomodulators of plant origin.
        Planta Medica. 1989; 55: 339-348