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Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice

      Abstract

      Background

      Several plants are traditionally used as birth control agents by the rural people in India. Mimosa pudica is one of the folk medicinal plants commonly used as antifertility agent in some places in India. The present work was carried out to evaluate the claimed antifertility effect of the plant by carrying out pharmacological studies with the root extract of the plant.

      Study Design

      Air-dried roots of M. pudica were extracted using methanol. Dried methanol extract of the root was administered orally to Swiss albino mice for 21 consecutive days. Estrous cycle, reproductive hormones (LH, FSH, prolactin, estradiol and progesterone) and number of litters produced were studied in both control and extract-administered groups by using standard methods. Phytochemical studies of the methanolic root extract were carried out using qualitative and thin-layer chromatography methods.

      Results

      M. pudica root extract, when administered orally at a dose of 300 mg/kg body weight/day, prolonged the length of the estrous cycle with significant increase in the duration of the diestrous phase and reduced the number of litters in albino mice. The number of litters was increased in the posttreatment period. The analysis of the principal hormones (LH, FSH, prolactin, estradiol and progesterone) involved in the regulation of the estrous cycle showed that the root extract altered gonadotropin release and estradiol secretion.

      Conclusions

      The root extract of M. pudica has antifertility effect as it prolongs the estrous cycle and disturbs the secretion of gonadotropin hormones in albino mice. The decrease in FSH level in the proestrus and estrus stages in the extract-administered group compared with those of control animals indicates the disturbance of estrous cycle and ovulation through suppression of FSH.

      Keywords

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      References

        • Bhattacharjee S.
        • Tiwari K.
        • Majumdar R.
        • Misra A.K.
        Folklore medicine from district Kamrup (Assam).
        Bull Med-Ethno-Bot Res. 1980; 1: 447-460
        • Tiwari K.C.
        • Majumder R.
        • Bhattacharjee S.
        Folklore information from Assam for family planning and birth control.
        Internal J Crude Drug Res. 1982; 20: 133-137
        • Aalok P.K.
        Lajjalu ��� an indispensable drug for blood pressure.
        Sacitra Ayurveda. 1997; 51: 21-22
        • Nayagam M.C.
        • Pushparaj M.S.
        Touch me not: a medicinal plant of the Nilgiri tribals ��� a study.
        J Econ Taxon Botany. 1999; 23: 417-420
        • Valsala S.
        • Karpagaganapathy P.R.
        Effect of Mimosa pudica root powder on oestrous cycle and ovulation in cycling female albino rat Rattus norvegicus.
        Photother Res. 2002; 16: 190-192
        • Krebs K.G.
        • Heusser D.
        • Wimmer H.
        Spray reagents.
        in: Stahl E. Thin layer chromatography. Springer Verlag, Berlin1969: 854-909
        • Harborne J.B.
        Phytochemical methods. A guide to modern techniques of plant analysis.
        2nd ed. Chapman and Hall, London1984
        • Makonnen E.
        • Rostom A.H.
        • Assefa G.
        • Zerihun L.
        Antifertility effect of Jatropha curcas L seed in guinea pigs.
        Ethiop J Health Dev. 1997; 11: 145-148
        • Salhad A.S.
        • Issa A.A.
        • Alhougog I.
        On the contraceptive effect of castor beans.
        Indian J Nat Med. 1997; 35: 63-65
        • Gupta S.
        Sampling and test of significance.
        in: Gupta S. Statistical methods. Sultan Chand and Sons, New Delhi1978: 58-76
        • Gebrie E.
        • Makonnen E.
        • Debella A.
        • Zerihun L.
        Phytochemical screening and pharmacological evaluations for the antifertility effect of the methanolic root extract of Rumex steudelii.
        J Ethnopharmacol. 2005; 6: 139-143
        • Ganguly M.
        • Borthakur M.K.
        • Devi N.
        • Mahanta R.
        Antifertility activity of the methanolic leaf extract of Cissampelos pareira in female albino mice.
        J Ethnopharmacol. 2007; 111: 688-691