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Abstract
Aristolic acid (AA), obtained from Aristolochia indica Linn, disrupted nidation in mice when administered on Day 1 of pregnancy. The implantation
inhibiting effect of the compound was assessed with respect to certain paramenters
which are characteristics of early pregnancy, such as tubal transport of ova into
the uterus, hyperpermeability of the endometrial capillaries, increase in uterine
weight and total protein content, endometrial bed preparation and changes in uterine
phosphatase enzymes during Days 4–6 of pregnancy. The compound did not affect tubal
transport of eggs, but the uterine blue reaction, caused by extravasation of the dye,
pontamine blue, at future implanation sites was inhibited significantly in treated
mice. Histological picture of the uterus revealed AA-induced impairment of development
(i.e. decidualization) and reconciled with decreases found in uterine weight and its
total protein contents in treated animals. In control untreated mice, specific uterine
alkaline phosphatase (ALP) activity increased significantly from Days 4 through 6
of pregnancy, but this was prevented in treated mice. On the other hand, specific
uterine acid phosphatase (AP) activity was high on Day 5, while in treated mice uterine
AP activity remained low during Days 4 and 5 and increased significantly thereafter.
It was inferred that AA interferes with steroidal conditioning of the uterus and renders
it hostile to ovum implantation.
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References
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Article info
Publication history
Accepted:
December 1,
1986
Received:
November 6,
1986
Identification
Copyright
© 1987 Geron-X, Inc. Published by Elsevier Inc. All rights reserved.