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Disruption of pregnancy in mouse by Aristolic acid: I. Plausible explanation in relation to early pregnancy events

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      Abstract

      Aristolic acid (AA), obtained from Aristolochia indica Linn, disrupted nidation in mice when administered on Day 1 of pregnancy. The implantation inhibiting effect of the compound was assessed with respect to certain paramenters which are characteristics of early pregnancy, such as tubal transport of ova into the uterus, hyperpermeability of the endometrial capillaries, increase in uterine weight and total protein content, endometrial bed preparation and changes in uterine phosphatase enzymes during Days 4–6 of pregnancy. The compound did not affect tubal transport of eggs, but the uterine blue reaction, caused by extravasation of the dye, pontamine blue, at future implanation sites was inhibited significantly in treated mice. Histological picture of the uterus revealed AA-induced impairment of development (i.e. decidualization) and reconciled with decreases found in uterine weight and its total protein contents in treated animals. In control untreated mice, specific uterine alkaline phosphatase (ALP) activity increased significantly from Days 4 through 6 of pregnancy, but this was prevented in treated mice. On the other hand, specific uterine acid phosphatase (AP) activity was high on Day 5, while in treated mice uterine AP activity remained low during Days 4 and 5 and increased significantly thereafter.
      It was inferred that AA interferes with steroidal conditioning of the uterus and renders it hostile to ovum implantation.
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      References

        • Pakrashi A.
        • Chakraborty B.
        Anti-estrogenic and anti-implantation effect of aristolic acid from Aristolochia indica (Linn).
        Ind. J. Exptl. Biol. 1978; 16: 1283-1285
        • Pakrashi A.
        • Chakraborty B.
        Biological properties of interceptive agents from Aristolochia indica (Linn.).
        Ind. J. Med. Res. 1977; 66: 991-998
        • Pakrashi A.
        • Chakraborty B.
        Antifertility effect of aristolic acid from Aristolochia indica (Linn) in female albino rabbits.
        Experientia. 1978; 34: 1377
        • Pakrashi A.
        • Ganguly T.
        Changes in uterine phosphatase levels in mice treated with aristolic acid during early pregnancy.
        Contraception. 1982; 26: 635-643
        • Bessey O.A.
        • Lowry O.H.
        • Brock M.J.
        J. Biol. Chem. 1946; 164 (cited in): 331Bergmeyer H.U. Methods of Enzymatic Analysis. Academic Press, New York1965: 783-785
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265-267
        • Humphrey K.W.
        The antifertility actions of some antiestrogens in the mouse.
        in: Litonjua A.D. Proceedings of the Third Asia and Oceania Congress of Endocrinology. 1967: 784-789
        • Finn C.A.
        The biology of decidual cells.
        Adv. Reprod. Physiol. 1971; 5: 1-26
        • Psychoyos A.
        The hormonal interplay controlling egg implantation in the rat.
        in: McLaren A. Advances in Reproductive Physiology. Logos Press, New York1969: 257-277
      1. (Part II)
        • Psychoyos A.
        Endocrine control of egg implantation.
        in: Greep R.O. Astwood E.B. Handbook of Physiology. vol. II. American Physiological Society, London1963
        • Malinowska K.W.
        • Fotherby K.
        Enzymic activity in rat uterus during early pregnancy.
        Hormone Res. 1975; 6: 95-104
        • Murdoch R.N.
        • Kay D.J.
        • Cross M.
        Activity and subcellular distribution of mouse uterine alkaline phosphatase during pregnancy and pseudopregnancy.
        J. Reprod. Fert. 1978; 54: 293-300
        • Parthasarthy G.
        • Purandare T.
        • Katrak B.
        • Juneja H.S.
        • Munshi S.R.
        Changes in uterine phosphatase levels in mice deprived of LH during early pregnancy.
        J. Reprod. Fert. 1979; 56: 297-300
        • Finn C.A.
        • Hinchliffe J.R.
        Reaction of the mouse uterus during implantation and deciduoma formation as demonstrated by changes in the distribution of alkaline phosphatase.
        J. Reprod. Fert. 1964; 8: 331-338
        • Manning J.P.
        • Carter S.G.
        • Butler M.C.
        Artificial stimulatory and hormonal requirements of decidual ALP in pseudopregnant rat.
        Acta. Endocr. Copenh. 1969; 61: 164-172
        • Smith M.S.R.
        • Wilson I.B.
        Histochemical observations on early implantation in the mouse.
        J. Embryol. Exp. Morphol. 1971; 25: 165-174
        • Lobel B.L.
        • Levy E.
        • Linder H.R.
        Cytochemical studies of oviducts on the rat during the estrous cycle and progestation.
        in: Raspe G. Scherring Symposium on Advances in the Bio-Sciences. vol. 4. Pergamon Press, 1970: 299-322
        • Finn C.A.
        • Martin L.
        The role of estrogen secreted before oestrus in the preparation of the uterus for implantation in the mouse.
        J. Endocr. 1970; 47: 431-438
        • Kabir S.N.
        • Pal A.K.
        • Pakrashi A.
        Inhibitory influence of aristolic acid on uptake of 2,4,6,7-3Hestradiol by mouse uterus.
        IRCS Med. Sci. 1981; 9: 329
        • Pal A.K.
        • Kabir S.N.
        • Pakrashi A.
        Aristolic acid, an inhibitor of estrogen-induced progesterone uptake by castrated mouse uterus.
        IRCS Med. Sci. 1981; 9: 481-482