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Abstract
Gossypol has been shown to inhibit steroidogenesis in leydig cells. This study examined
the mechanism of this action by investigating the effect of gossypol on leydig cell
hCG receptor binding, adenylate cyclase activity and cyclic AMP production in leydig
cells. Gossypol had no effect on hCG binding to cell membranes but inhibited LH-stimulated
cyclic AMP production in whole purified leydig cells. It also reduced the stimulation
caused by LH, Gpp(NH)p, forskolin and fluoride in membranes from leydig cells and
also from liver. It is therefore possible that gossypol affects cyclic AMP production
at the level of ATP conversion to cyclic AMP, although effects on the G-protein and
catalytic subunit cannot be ruled out.
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References
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Article info
Publication history
Accepted:
December 4,
1986
Received:
October 15,
1986
Identification
Copyright
© 1987 Geron-X, Inc. Published by Elsevier Inc. All rights reserved.
