Abstract
Changes in the physical characteristics of semen and ultrastructure of the spermatozoa
of langur monkeys after vas occlusion with styrene maleic anhydride (SMA), a polymer
with pH-lowering action, are reported. Vas occlusion resulted in severe reversible
hypospermia. Severe oligospermia was observed in the majority of animals (five of
eight) in the first ejaculation, 30 days after vas occlusion, and in two animals in
the second ejaculation, 60 days after vas occlusion. Subsequent monthly ejaculations
for 5 months revealed uniform azoospermia. The voided spermatozoa were immotile and
supravital staining confirmed necrospermia. Scanning electron microscopy (SEM) revealed
severe coiling of tail, rupture of acrosomal envelope, and bent midpiece associated
with damaged mitochondrial sheath. Observations by transmission electron microscopy
(TEM) revealed vacuolization in the nucleus, membrane damage in the acrosome, loss
of segmented columns, and numeric aberrations in the centriole of the neck, as well
as degeneration of mitochondrial sheath and axoneme in the midpiece, and absence of
outer plasma membrane in the midpiece and tail. The results indicate that the necrospermic
status of the spermatozoa during initial ejaculations may offer instant sterility
after vas occlusion with SMA.
Keywords
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Article info
Publication history
Accepted:
January 5,
1998
Received in revised form:
December 23,
1997
Received:
October 23,
1997
Identification
Copyright
© 1998 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
