Original Research Articles| Volume 57, ISSUE 2, P125-132, February 1998

Ultrastructural Changes in the Spermatozoa of Langur Monkeys Presbytis entellus entellus After Vas Occlusion With Styrene Maleic Anhydride


      Changes in the physical characteristics of semen and ultrastructure of the spermatozoa of langur monkeys after vas occlusion with styrene maleic anhydride (SMA), a polymer with pH-lowering action, are reported. Vas occlusion resulted in severe reversible hypospermia. Severe oligospermia was observed in the majority of animals (five of eight) in the first ejaculation, 30 days after vas occlusion, and in two animals in the second ejaculation, 60 days after vas occlusion. Subsequent monthly ejaculations for 5 months revealed uniform azoospermia. The voided spermatozoa were immotile and supravital staining confirmed necrospermia. Scanning electron microscopy (SEM) revealed severe coiling of tail, rupture of acrosomal envelope, and bent midpiece associated with damaged mitochondrial sheath. Observations by transmission electron microscopy (TEM) revealed vacuolization in the nucleus, membrane damage in the acrosome, loss of segmented columns, and numeric aberrations in the centriole of the neck, as well as degeneration of mitochondrial sheath and axoneme in the midpiece, and absence of outer plasma membrane in the midpiece and tail. The results indicate that the necrospermic status of the spermatozoa during initial ejaculations may offer instant sterility after vas occlusion with SMA.


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        • Guha SK
        • Ansari S
        • Anand S
        • Misro MM
        • Farooq A
        • Sharma DN
        Contraception in the male monkey by intra vas deferens injection of a pH-lowering polymer.
        Contraception. 1985; 32: 109-118
        • Guha SK
        • Singh G
        • Anand S
        • Ansari S
        • Kumar S
        Phase I clinical trial of an injectable contraceptive for the male.
        Contraception. 1993; 48: 365-375
        • Guha SK
        Injectable contraceptives for the male.
        Exp Opin Invest Drugs. 1995; 4: 537-543
        • Sethi N
        • Srivastava RK
        • Singh RK
        Chronic toxicity of styrene maleic anhydride, a male contraceptive in rhesus monkeys.
        Contraception. 1990; 42: 337-348
      1. Sharma RS. Reproductive endocrine and exocrine profiles of the male and female langurs Presbytis entellus entellus Dufresne. Ph.D. Thesis, Jaipur, India: University of Rajasthan, 1983.

        • David GFX
        • Ramaswami LS
        Reproductive system of North Indian langur (Presbytis entellus entellus Dufresne).
        J Morphol. 1971; 135: 99-130
        • Lohiya NK
        • Sharma RS
        • Puri CP
        • David GFX
        • Anand Kumar TC
        Reproductive exocrine and endocrine profiles of female langur monkeys, Presbytis entellus entellus.
        J Reprod Fertil. 1988; 82: 485-492
      2. Guidelines for Care and Use of Animals in Scientific Research. Indian National Science Academy, New Delhi1992
      3. Guha SK. Contraceptive for use by a male. USA Patent 1996, 5488075.

      4. WHO laboratory manual for examination of human semen and sperm-cervical mucus interaction. Cambridge University Press, New York1992
        • Guha SK
        Male fertility regulation by intra vas deferens injections.
        in: Moudgal NR Yoshinaga K Rao AJ Adiga PR Perspectives in primate reproductive biology. Wiley Eastern, New Delhi1991: 345-351
        • Guha SK
        • Singh G
        • Ansari S
        • Kumar S
        • Srivastava A
        • Koul V
        • Das HC
        • Malhotra RL
        • Das SK
        Phase II clinical trial of a vas deferens injectable contraceptive for the male.
        Contraception. 1997; 56: 245-250